Concentrating Solution, 50ml
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    Concentrating Solution, 50ml

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    DESCRIPTION: Sample concentration solution for Chondrex, Inc.’s Sirius Red Total Collagen Detection Kit (Cat # 9062) 

    QUANTITY: 50 ml 

    FORM: Polyethylene glycol solution

    USAGE: Collagen levels in culture media are generally lower than the standard range of the Sirius Red Total Collagen Detection Kit (Cat # 9062). This concentrating solution (Cat # 90626) and the following protocol can be used for concentrating culture media samples. 

    1. Take 1 ml culture medium.

    2. Add 250 µl of Concentrating Solution

    3. Vortex and incubate at 4°C for 16-24 hours.

    4. Centrifuge at 10,000 rpm for 3 minutes. 5. Discard supernatant.

    6. Add 100 µl of 0.05M acetic acid to dissolve the pellet. Use this solution as your sample.

    7. The calculated collagen concentration should be multiplied by a 0.1 dilution factor

    STORAGE: -20°C

    STABILITY: 2 years

    NOTES: N/A