HydraGreen™ Safe DNA Dye - 1ml (400x 50ml or 200x 100ml agarose gels)

(0 review)

404.00 € 404.0 EUR 404.00 € VAT Excluded

404.00 € VAT Excluded

Not Available For Sale

    This combination does not exist.

    Terms and Conditions
    30-day money-back guarantee
    Shipping: 2-3 Business Days

    HydraGreen™, 20,000X in water, is a non-carcinogenic and non-toxic alternative to Ethidium bromide used for the

    detection of nucleic acids in agarose gels. It is as sensitive as Ethidium bromide. There is also no toxic DMSO as HydraGreen™ is supplied in water.

    HydraGreen™ has fluorescence excitation maxima at 295 nm and 490 nm. The fluorescence emission maxima is similar

    to EtBr when bound to DNA – at 530 nm.


    Note: It is highly recommended agarose gel to be ≤0.5cm in thickness. TAE buffer system is preferred for higher



    Wait for melted agarose to be cooled to 55ºC.

    Add 4-6 µl per 100 ml of agarose or 2-3 µl per 50 ml of agarose, swirl and mix well.

    IMPORTANT : HydraGreen ™ is supplied in 20,000X concentration in water



    Use 10 to 15 µl per 100 ml of staining solution. Same as when using ethidium bromide. For an average gel thickness of

    about 0.5 cm, stain 30 minutes, followed by a destain of 30 minutes in water. Protect gel and staining solution from

    light with aluminum foil or place in dark.


    Detect bands under UV illumination (yellow or green gelatin- or cellophane filters is recommended for clearer bands) or

    non-UV LED illuminators such as Blue Light LED illumination.