Detect Range: 3.12-200 ng/mL
Sensitivity: 1.2 ng/mL
Target Protein Name: Human Mast/stem cell growth factor receptor
ELISA type: Sandwich ELISA Kit
Product Description: Human Kit ELISA Kit allows for the in vitro quantitative determination of Human Mast/stem cell growth factor receptor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Kit ELISA Kit has been pre-coated with an antibody specific to Human Mast/stem cell growth factor receptor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Mast/stem cell growth factor receptor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Mast/stem cell growth factor receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.