Human NEFL (Neurofilament, Light Polypeptide) Competitive ELISA kit - 96 wells plate

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    Product name:Human NEFL(Neurofilament, Light Polypeptide) ELISA Kit
    Reactivity:Human
    Alternative Names:CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
    Assay Type:Competitive Inhibition
    Sensitivity:46.4 pg/mL
    Standard:10000 pg/mL
    Range:156.25-10000 pg/mL
    Sample Type:serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
    Assay Length:2h
    Research Area:Neuro science;
    Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NEFL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEFL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEFL in the samples is then determined by comparing the OD of the samples to the standard curve.


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