| Reactivity: | Panspecies |
| Alternative Names: | VA, Vit. A |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 9.38 ng/mL |
| Standard: | 1000 ng/mL |
| Detection Range: | 15.63-1000 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Metabolic pathway; Endocrinology; Hormone metabolism; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VA in the samples is then determined by comparing the OD of the samples to the standard curve. |