[AFG-E9108] AffiELISA® Human L-Carnitine ELISA Kit - 96-wells plate

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    Product name:Human L-Carnitine ELISA Kit
    Reactivity:Human
    Alternative Names:L-Carnitine
    Assay Type:Sandwich
    Sensitivity:0.115 ng/mL
    Standard:20 ng/mL
    Detection Range:0.32-20 ng/mL
    Sample Type:serum, plasma, urine, tissue homogenates
    Assay Length:3.5h
    Research Area:Biochemicals
    Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human L-Carnitine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human L-Carnitine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human L-Carnitine in the samples is then determined by comparing the OD of the samples to the standard curve.

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